WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE DETERMINATION OF TULOBUTEROL IN TRANSDERMAL DRUG DELIVERY SYSTEM

Kavita Parui* and Dr. Snehalatha Boddu

Abstract

A sensitive, accurate and reproducible RP-HPLC method has beendeveloped and validated for the determination of Tulobuterol intransdermal drug delivery system. Chromatographic separation wasachieved isocratically on a Prontosil C18 column (250 x 4.6 mm, 5 μm)with a mobile phase consisting of Acetonitrile:0.02M Potassiumdihydrogen phosphate buffer (60:40), adjusted to pH 3.0 withOrthophosphoric acid at the flow rate of 1.0 mL/min. UV detectionwas performed at 215 nm. The retention time for tulobuterol was foundto be 2.880 minute. The proposed method was validated by parameterssuch as selectivity and specificity, linearity, accuracy, precision, limitof detection, limit of quantification, robustness and assay according to the ICH guidelines.The calibration curve of tulobuterol was linear in the range of 25 – 75 μg/mL with correlationcoefficient (r2) of 0.9998. Both intra and inter-day precision and accuracy were withinacceptable limits. The limit of detection and limit of quantification for tulobuterol were foundout to be 2.90 and 0.95 respectively. The robustness was also estimated on the smallfluctuations in the mobile phase compositions, wavelength and the flow rate. The developedmethod can be applicable for routine quantitative analysis.

Keywords: Tulobuterol, Transdermal drug delivery, RP-HPLC, Method Validation.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More