Abstract

ANTIOXIDANT, CYTOTOXIC AND PHYTOCHEMICAL ASSESSMENT OF LEAF EXTRACTS OF GOLDEN TRUMPET (ALLAMANDA CATHARTICA L.)

Rashmi Das and Sunita Bhatnagar*

Abstract

Medicinal plants have been used since time immemorial for a number of diseases. Phytochemical analysis of medicinal plants gives an insight towards its medicinal potential. In the present study leaf extracts of one flowering plant Allamanda cathartica was explored for its phytochemical, cytotoxic and antioxidant activity. On phytochemical analysis Flavonoids and saponins were present in chloroform, acetone and methanol extracts. Terpenoids was present in hexane, chloroform and acetone extract. Tannin was present in fresh, acetone and methanol extracts. Cardiac glycoside was present in hexane, acetone and methanol extract. Highest concentration of phenolic was found in the acetone extract of the plant 124.23μg(GAE)/ g followed by methanol extract with 92.07μg(GAE)/ g. Highest concentration of flavonoids was found in the acetone extract of the plant 605.50μgQE/g followed by chloroform extract with 307.16μgQE/g. Cytotoxic activity was significant in chloroform extract 97.97% at the dose 200μg/ml followed by hexane extract which showed 77.5 % activity at the highest dose. Remaining extracts showed only mild activities against brine shrimp mortality assay. Highest FRAP value was obtained in methanol extract followed by acetone extract. However extracts showed mild activity in DPPH radical scavenging assay. Results indicated in the study points towards a significant medicinal potential of leaf extracts of Allamanda cathartica.

Keywords: Anti oxidant activity, Phytochemical analysis, Brine shrimp mortality assay.