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WJPR

World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

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Abstract

DEVELOPMENT AND VALIDATION OF VISIBLE METHOD FOR ESTIMATION OF AMPICLLIN IN BULK FORMULATION

Chavi Dagar*, G. Rohit Reddy and T. Ramesh

Abstract

A simple, sensitive, accurate, precise and economical visible Spectrophotometric method was developed and validated for the estimation of Ampicillin in Bulk form. The method is based on the reaction of Ampicillin with FC Reagent [Folin Ciocalteu] in the presence of 10% Sodium Carbonate giving greenish blue colour chromogen which shows maximum absorbance at 720nm against reagent blank. The Chromogen obeyed Beer’s law in the concentration range of 10-50 μg/ml for Ampicillin. The results of the analysis have been validated statistically and recovery studies.

Keywords: Ampicillin, Folin Ciocalteu (Fc) reagent, Visible Spectrophotometric.

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