Abstract

MOLECULAR DETECTION OF CLASSICAL STAPHYLOCOCCAL SUPERANTIGENIC TOXIN GENES IN STAPHYLOCOCCUS AUREUS ISOLATES AND EFFECT OF PARTIAL PURIFIED TOXIN ON B6 MELANOMA CANCEROUS CELLS

Bareq A. Alajealy, *Maysaa S. Alshukri and Hassan S. Aljumaily

Abstract

The current study aimed to molecular detection of classical staphylococcal super antigenic toxin genes by identify Staphylococcus aureus isolated from stool samples in Iraqi governorate, Babylon city, A total of 100 specimens were collected from patients, between July 2016 and September 2016. 20 strains were isolated and identified by microbiological tests, At least one type of S. aureus classical enterotoxin gene (SEs) was harboring in all 20 (100%) of the isolates. The Polymerase chain reaction (PCR) used for detection of virulence genes that coding to enterotoxin, exfolative toxin and toxic shock syndrome toxin of S. aureus isolates, the result show that they were positive for, sea, seb, tst, eta, etb and agr with number and percentage, 8(40%),7(35%),20(100%),13(65%), 9(45%),14(70%) and 19(95%) respectively. many S. aureus isolates carry at least one of the enterotoxin genes and (95%) strains harbored more than one toxin coding genes. Also the results show effect of partially purified of S.aureus toxin on B16 melanoma as a model for human skin cancers, It has been revealed that S. aureus enterotoxin have an inhibiter effect on these cells.

Keywords: S. aureus, sea, seb, tst, eta, etb and agr.