Abstract

EVALUATION OF ANTI-DIARRHEAL PROPERTY OF CRUDE EXTRACTS AND DIFFERENT FRACTIONS OF STEM BARK AND FRUITS OF OROXYLUM INDICUM

Md. Mahfuzur Rahman, Majedul Islam*, Syed Abu Hasnat, Md. Rasal Hossain Khan, Nazmul Hasan Sumon and Dr. Md. Abdul Muhit

Abstract

Globally one in nine children deaths occur due to diarrheal diseases, making diarrhea the second leading cause of death among children under the age of five.[1] Specially, for Third World countries, millions of people are dying of it every year.[2] From the ancient time plants have been using as source of new drugs.[3] In this experiment, crude Methanolic extract of Oroxylum indicum bark and fruits with different soluble parts were subjected to investigate for the evaluation of antidiarrheal activity on mice in vivo experiment. Twenty eight adult Swiss albino mice (mus domesticus) were divided into seven groups for the experiment. Each group received a particular treatment i.e. control, standard, single dose of Methanolic crude extract and its different fractions of bark and fruits of Oroxylum indicum. Castor oil was used for the induction of diarrhea in mice. The methanolic crude extract and its different fractions (Ethyl acetate fraction, Dichloromethane fraction, Hexane fraction, Carbon tetrachloride fraction) (400mg/kg) cause reduction of diarrheal feces by 50%, 31.4%, 52.3%, 34.9%, 53.551.2%. The methanolic crude extract of fruit and its different fractions (Ethyl acetate fraction, Dichloromethane fraction, Hexane fraction, Carbon tetrachloride fraction) (400mg/kg) cause reduction of diarrheal feces by 46.5%, 32.6%, 45.3%, 33.7%, 51.2%. This experiment reveals that methanol extracts of bark and fruits of Oroxylum indicum showed significant anti-diarrheal property.

Keywords: Diarrhea, Oroxylum indicum, Swiss albino mice, methanolic extract.