Abstract

ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL FROM THE LEAVES AND STEMS OF MURRAYA KOENIGII

Zafar Iqbal*, Hafiz Khalid Mehmood, Mustansar Hussain, Mian Habib Ur Rehman Mehmood, Mohammad Naeem Choudhry

Abstract

Leaves and stems of murraya koengii were collected from the PCSIR locality and their essentials oils were extracted through hydrodistillation using Dean Stark apparatus. Extracted both oils were dried over anhydrous sodium sulfate and 0.17% and 0.06% yields were obtained for leaves and stems respectively. Their GC-MS analysis was performed for chemical constituents. Major constituents in the essential oil of leaves were α-pinene 2.7%, spathulenol 19.10%, caryophyllene 18.4%, caryophyllene oxide 17.6%, α-caryophyllene 10.2%, β-eleminene 9.1%, and germacerene 4.3% and while in the stem essential oil major constituents were α-pinene 4.0%, β-terpineol 2.9%, α-caryophyllene 7.2%, epiglubolol 5.4%, spathulenol 6.6%, clarene oxide 1.2%, heptatrioctanol 2.3% and phytol 1.9% respectively. Their antioxidant activity of both essential oils was measured by using DPPH and it was concentration dependant. Maximum activity was found at 100 μl was 78% and 59% for essential oil of leaves and stem respectively as compared to ascorbic acid.

Keywords: Murraya Koenigii, Essential oil, Hydro-distillation, GC-MS analysis, Caryophyllene, Spathulenol.