Abstract

IN VITRO ANTI-SKIN AGEING BENEFITS OF CLERODENDRUM PANICULATUM LEAF EXTRACTS.

Dilmel Krishnan R.*, Vijayakumar M., Sandeep Varma R., Ilavarasu A. and Dhanabal S. P.

Abstract

Clerodendrum paniculatum (Family: Lamiaceae) or Hanuman kireetam is a well-known ayurvedic plant but less explored in India. The objective of the present study was to check the anti-skin ageing benefits of methanol and water extracts of C. paniculatum leaves In vitro. The Collagen-I, Elastin and Hyaluronidase assays was performed on Human dermal fibroblast cells (HDF).The antioxidant potential of C. paniculatum leaf water (CPW) and methanol extracts (CPM) was checked by reducing power assay. The CPM and CPW extract was non-toxic to the cells with CTC50 above 200μg/mL. CPM and CPW showed good anti-oxidant activity comparable to Ascorbic acid at 200μg/mL. The Collagen and Elastin content was increased in the extract treated cells as compared to control. The Hyaluronidase inhibition confirmed increased hydration in cells for CPM and CPW respectively. HPLC chromatogram profile of CPM and CPW was developed using Acetonitrile: water as mobile phase in the ratio of 70:30. This study confirmed the presence of flavonoids, Quercetin and Rutin, as biomarkers in CPM extracts and CPW. Both water and methanol extract showed good skin anti-ageing activity in HDF .The study overall substantiates our objective of anti-skin ageing activity of CPW and CPM leaf extracts, and can be explored as potential ingredient in skin care formulations.

Keywords: Clerodendrum paniculatum, Quercetin, Rutin, Anti-ageing.