Abstract

IDENTIFICATION OF ANTIOXIDANT COMPOUND CHOLEST-5-EN- 3-OL FROM CHLOROFORM EXTRACT OF GRACILARIA FOLIIFERA USING GC-MS ANALYSIS

Jenifer P., C. P. Balakrishnan* and S. Chidambaram Pillai

Abstract

The present study deals with identification and evaluation of antioxidant compound Cholest-5-en-3-ol by using GC-MS analysis from chloroform extract of marine red algae Gracilaria foliifera J. Agardh of Manapad coast, Tamil Nadu. In-vitro antioxidant analysis like DPPH, H2O2, OH, NO radical and total antioxidant activity also carried out by using standard methods. The GC-MS analysis has shown the presence of different phytochemical compounds in the chloroform extract of G. foliifera. Over 30 phytochemical compounds have been identified, among these, the phytochemical compound Cholest-5-en-3-ol with retention time (RT) 31.79 min and peak area 5.08% (Molecular formula C27H46O and Molecular weight 386). The similar studies from plants were agreed that compound Cholest-5-en-3 -ol is activity of antioxidant. In-vitro antioxidant result indicated that chloroform extract of G. foliifera exhibited significant value were compared with standard.

Keywords: Seaweed, G. foliifera, phytochemical screening, GC-MS, in-vitro Antioxidant.