Abstract

IN VIVO AND IN VITRO PRODUCTION OF FLAVONOIDS FROM CALLUS CULTURES OF BUTEA MONOSPERMA

Chandkor Kumari and *Renu Sarin

Abstract

Flavonoids are antioxidants present in many herbal plants. A protocol for the production of three important flavonoids namely Kaempferol, Quercetin and Luteolin from various plant parts and in vitro grown callus cultures of a medicinally important plant of family fabacece viz. Butea monosperma was developed. Butea monosperma is popularly called as “dhak” or “palas” and commonly known as “Flame of forest”. The crude extracts of various plant parts of this plant possess antibacterial, antifungal, hypoglycemic and anti-inflammatory activities Kaemferol, Quercetin and Luteolin are three important flavonoids which have been isolated and identified from plant parts viz. seeds, leaves, flowers and unorganized callus cultures of Butea monosperma raised and maintained by frequent subculturing on Murashige and Skoog’s medium (1962) supplemented with various growth regulators. Quantification data revealed that the total amount of free flavonoids and bound flavonoids was higher in callus as compared to plant parts and the amount of free flavonoids is more than bound flavonoids both in vivo plant parts and in vitro callus cultures.

Keywords: Kaempferol, Quercetin, Luteolin, Tissue culture, Butea monosperma.