Abstract

STUDY OF ANTIMICROBIAL ACTIVITY OF BETEL LEAVES EXTRACTS AGAINST ESBL AND MBL PRODUCING PATHOGENES

*Kalpana Tilak and Tulika Mishra

Abstract

Bacterial resistance to antibiotics is an emerging problem of today’s world. Studies on herbal and Ayurvedic extracts are done in order to treat the infections caused by uropathogens. The aim of the study was to assess the anti microbial activity of piper betle leaf extract on ESBL and MBL producing uropathogens under in-vitro conditions. Piper betle is a plant which exhibits medicinal properties. The active components of piper betle were extracted for 12hours at 60°C with the help of Soxhlet apparatus using ethanol, methanol, water and acetone as solvents. The above solvents extracts were concentrated at 40°C on water both to obtain a semi-solid mass which was used to carry out qualitative and quantitative analysis. The antimicrobial activity of ethanol, methanol, acetone and water extracts were tested against ESBL and MBL producing uropathogens i.e.41 escherichia coli, 10 proteus, 9 Citro, 12 Pseudomonia and 17 Klebsiella pneumonia. The ethanol and methanol extract showed inhibition on all these cultures by agar well diffusion method and the zone size ranged from 15-25mm. the minimum bactericidal concentration (MBC) for ethanol extract was found to be 10mg/ml. the significance of the study was conducted to investigate the in – vitro anti microbial activity of Piper betle plant against antibiotic resistant uropathogens.

Keywords: Betel Leaves, Piper Betel Plant, Uropathogenes.