Abstract

NEW IDENTIFICATION OF PHAGES IN WATER AND IN FISHES FROM LAGOON EBRIÉ AND THEIR APPLICATION FOR MULTIDRUG STRAINS IN ABIDJAN, WEST AFRICA

Kakou Ngazoa E. Solange*, Kouya Dominique, Koudou A. Aristide, Abole Ahmed, Guessend K. Nathalie, Sylla Aboubacar, Sina Kouamé Mireille, Coulibaly Ngolo David, Kouassi Kan Stéphane, Meite Syndou, Aoussi Serge and Dosso Mireille

Abstract

Phages are widely apply to screen, to detect and to eliminate bacterial in different applications. In Africa, the emergence of antibiotic resistance strains and the fecal contamination in the aquatic environment require to explore the phage therapy. In Abidjan, the Lagoon Ebrié is an important aquatic ecosystem and the increase of the microbial contamination represents a risk for the population and the fisheries activity. The aim of this study is to identify novel phages in water and in fish samples from the Lagoon and to test their virulence against multidrug resistant strains. Water samples from different lagoon sites were collected and analyzed in culture and phages test assay. Fresh fishes were collected from Lagoon’s fish market in Abobodoumé/Locodjoro site and PWLO6, PWR7 and PWAD8 analyzed by extraction of intestinal organs. Water samples and intestinal solution were passed through 0.45 μm syringe filters and incubated in bacteria growth. The enrichment of bacteria and phages were done in LB media at 37°C. We have isolated 5 phages from different types of fishes and 3 phages from Lagoon sites. The phages of fishes PFSO1, PFMU2, PFCAR4 and PFCAP5 have positive virulence for all tested antibiotic multidrug resistant strains Pseudomonas aeruginosa, Salmonella sp, Enterobacter aerogenes, Klebsiella pneumoniae and E. coli. The phage PFMA3 has shown not effect against Klebsiella pneumoniae and Enterobacter aerogenes. 3 phages from water have identical virulence effect in all tested bacteria. This study report the first isolation of 8 novel phages in Lagoon Ebrié with virulent effect on multidrug resistant’s strains and can be apply as a new approach for eliminating of pathogens.

Keywords: Phages, aquatic ecosystem, fishes, Lagoon Ebrié, multidrug resistant strains, West Africa.