Abstract

SPECTROPHOTOMETRIC QUANTIFICATION OF FLAVONOID CONTENT IN HERBAL DRUGS EXTRACTS AND OPTIMIZATION OF MICROWAVE ASSISTED EXTRACTION TECHNIQUE BY USING DIFFERENT SOLVENTS.

Dr. Mohini Kuchekar*, Mohini Upadhye and Swati Chawan

Abstract

The quantitative determination of flavonoids in beans, leaves, roots, peels of Ficus glomerata, Citrus aurantium, Spinacea oleracea, Camellia sinensis and Phaseolus vulgaris respectively was carried out using spectrophotometric methods. Quercetin reagent was used as the standards for calibration of the flavonoids. The plant material was collected from local Pune, Maharashtra, India. In the present study the flavonoid content of ethanolic and aqueous roots, peels, leaves, beans extracts was evaluated by Aluminium chloride colorimetric method. Total flavonoid content in aqueous and ethanolic extract of Ficus glomerata (roots extract), Citrus aurantium (peels extract), Camellia sinensis (leaf extract), Spinacea oleracea (leaf extract) and Phaseolus vulgaris (seed extract) were found to be (169mg/gm, 623mg/gm), (52mg/gm, 102mg/gm), (126mg/gm, 208mg/gm), (520mg/gm, 458mg/gm) and (262mg/gm, 456mg/gm) respectively. The optimization of extraction conditions were performed using different solvents. It was concluded that ethanolic extract by microwave synthesis gives maximum amount of flavonoid content.

Keywords: Flavonoid content, Ficus glomerata, Citrus aurantium, Camellia sinensis, Spinacea oleracea, Phaseolus vulgaris.