Abstract

PHYTOCHEMICAL SCREENING AND IN-VITRO CORNEAL WOUND HEALING ACTIVITY OF THE LEAVES OF MANILKARA ZAPOTA (L) VAN ROYEN Var. PKM1 IN NEWER HERBAL DRUG DEVELOPMENT

Swarnakumari S.*, Periyanayagam K., Ida Christi V. E. and Mohan S.

Abstract

Research on wound healing is a developing area in modern biomedical science. A large number of plant / plant extract / decoctions or paste are equally used by tribal and folklore traditions in India for treatment of cuts, wounds and burns. Preliminary phytochemical screening was carried out with solvents of different polarities reveals the presence of carbohydrates, proteins and amino acids, flavonoids, terpenoids, tannins and phytosterols. Ethanolic extract of leaves indicates the presence of flavonoids while in other extract it seems to be negative. Developing in vitro bovine model, a new avenue for the exploration of newer drug development in ophthalmic diseases has been furnished. This system seems to be very rapid, economic and reliable. Study aims to reveal the significant wound healing dose dependent corneal wound healing effect of ethanolic extract of the leaves of Manilkara zapota (L). The mechanism of action may be due to the presence of bioflavonoid by its antioxidant property. In cornea, EGF epidermal growth factor is thought to be the main chemical mediator but in the skin no single factor has been identified as the major regulator. It is not clear whether upregulation of miotic activator (or) down regulation of a miotic inhibitor was responsible for the high level of cell division post wounding.

Keywords: Bioflavonoids, In-vitro, Ophthalmic, Wound healing, Ethanolic extract, M. zapota Leaves.