Abstract

ANTIBACTERIAL SCREENING OF NEWLY SYNTHESIZED MANNICH BASES DERIVED FROM 5H-DIBENZO[B,F]AZEPINE-5-CORBOXAMIDE AGAINST GRAM POSITIVE AND GRAM NEGATIVE PATHOGENS

Sheela Joshi and Kapil Vyas*

Abstract

Mannich bases are well known for their antimicrobial activities, In this study, Mannich bases 3a-3j (5H-dibenzo[b,f]azepine-5-Corboxamide methyl amines), were designed, synthesized, characterized and evaluated for antimicrobial screening.A series of Mannich bases of 5H-dibenzo [b,f]azepine-5-Corboxamidewere synthesised via reacting 5H-dibenzo [b,f]azepine-5-Corboxamide with secondary amines and sulphonamides. Structureevaluation of synthesized compounds wasdone on the basis of elemental analyses, UV, IR and 1HNMR spectral studies. The biological screening of these synthesized compounds was done against pathogenic microorganism Bacillus subtilis(Gram-positive bacteria) and Salmonella typhi (Gram-negativebacteria) with a view to explore their antimicrobial action bypaper disk method at 80, 160 and 320μg/ml respectively. The antibacterial activity of derived Mannich bases was compared against their parent sulphonamides. The results demonstrate the potential and importance of mounting new Mannich bases against pathogens.

Keywords: 5H-dibenzo[b,f]azepine-5-corboxamide, Sulphonamides, Amines, Mannich reaction, Mannich bases andAntimicrobial activity.