Abstract

ANTAGONISTIC EFFECT OF BACILUS SUBTILIS AGAINST PATHOGENICITY OF FUSARIUM SOLANI

Shaimaa Nghamish Mizil., *Intesar Ali Mezeal and Liqaa Jameel Ibraheem

Abstract

Antagonistic Activities of Bioagent against F.solani The bioagente Bacillus subtilis isolates were tested for antagonistic effect against F.solani on Petri dishes, all the investigated bioagentes isolates showed individual inhibition patterns towards the pathogenic fungus, and even but weakly in some isolates, this shows that antagonism is fungicidal in nature. On this basis, we selected the most efficient antagonistic isolates based on the criteria showing capacity of mycelia inhibition of at two fungi, resultes showed that among four isolates from B. subtilis isolates that B. subtilis 1 had greatly inhebition on the linear growth of F. solani. Results obtained in table 1 indicate that the isolate of F. solani was inhibited by B. subtilis1, by 73.2. comparing with the control. Under greenhouse conditions, B. subtilis were shown to be effective in reduction of damping off of tomato seedlings, when applied in mix with F. solani compared to soil infested only with the pathogens. Data presented in table 2 reveal that soil infested with treated soil with F. solani reduction of damping off of seedlings were more higher 66.5%. Results show that the efficiency of B. subtilis to antagonize F. solani under greenhouse conditions were 14.1 %. However, higher percentage in reduction of damping off of tomato seedlings was attained in response to treatment with dual bioagents B. subtilis than the individual one. It were 7.2% in soil infested with F. solani.

Keywords: F. solani, B. subtilis.