Abstract

A SUITABLE PROTOCOL FOR MICROPROPAGATION OF SPILANTHES CALVA DC. AN ENDEMIC MEDICINAL PLANT

*R. Rethinam and R. Jeyachandran

Abstract

An in vitro regeneration protocol has been standardized from nodal explants of Spilanthes calva DC. belongs to family Asteraceae. An endemic medicinal plant used for the treatment of stimulant and sialagogue, given in toothache, affections of throat and gums and paralysis of the tongue. MS medium supplemented with NAA (1.5 mg/L) and BAP (2.0 mg/L) was used to multiple shoots induction. The shoots were transferred in rooting MS medium supplemented with IAA (1.0 mg/L) and IBA (1.5 mg/L). Acclimatization was carried out on artificial soil with survival rate.

Keywords: BAP, IBA, NAA, and Spilanthes calva.