Abstract

SIMULTANEOUS ESTIMATION OF METHOTREXATE AND TRETINOIN BY REVERSE PHASE –HPLC METHOD

Aditya S., Arun P.*, Neelesh D., Neeraj K., Shailendra P., Shivani T. and Stuti P.

Abstract

A simple fast, precise and accurate liquid chromatographic method was developed for simultaneous estimation of Methotrexate(MTX) and tretioin(ATX) combined dosage form by RP-HPLC (Reverse Phase High Performance Liquid Chromatography) method. Chromatography was out on a column -18 and ACN:Buffer (85:15 v/v) of resultant pH 3.0, as mobile phase, at a flow rate of 1.2ml/min and measured at 340nmth. The calibration curves linearity range were 1-6ug/ml for methorexate and 1-16 ug/ml for tretioin, regression coefficient values are 0.9989 and 0.9999 respectively. The average retention time of Methotrexate and trition were found to be 1.95 and 8.98 min respectively. The method was reproducible, with good resolution and sharp peak of Methotrexate and tretioin. There results of the analysis have been validated statistically.

Keywords: METHOTREXATE, TRETIONIN, RP-HPLC, ASSAY, UV DETECTION.