Abstract

UTILIZATION OF BUTTERMILK PROCESSING WASTE FOR CEPHALOSPORIN C PRODUCTION BY A. CHRYSOGENUM NCIM 1069

Shruti Singh, Arpita Gupte and Sunita Singh*

Abstract

This study concerns the potential of dairy waste like buttermilk processing waste for Cephalosporin C production. The production, isolation and purification of Cephalosporin C by A. chrysogenum NCIM 1069 strain were studied. Assessment in the production of Cephalosporin C by manipulation of the dairy waste by medium formulation was used to arbitrate the strain performance. The buttermilk whey was supplemented with lactose and different organic and nitrogen sources and fermented at 30°C with agitation speed of 200 rpm for 168 hrs. The effect of different nitrogen source on Cephalosporin production was found to be insignificant. The highest Cephalosporin C (900μg/ml) was obtained from buttermilk supplemented with lactose, methionine and ammonium sulphate in cultures grown at 30°C after a period of 168 hrs. After a preliminary purification from medium using activated charcoal column, the bioassay using A. fecalis revealed presence and concentration of Cephalosporin. Further confirmation of the Cephalosporin was carried out by performing HPLC. This study is first report on utilization of dairy waste like buttermilk processing waste to effectively produce Cephalosporin.

Keywords: buttermilk waste, cephalosporin, methionine, antimicrobial activity.