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Abstract

MATHEMATICAL MODELING OF SOME MEDIUM CONSTITUENTS AFFECTS ON THE PRODUCTION OF VITAMIN B12 UNDER SOLID STATE FERMENTATION

*Ibrahim S. Abd-Elsalam, Mostafa M. El-Sheekh, Samya Shabana and Ahmed zaki

Abstract

The present investigation involved testing the effect of different carbon sources ( glucose , sucrose , and starch ) as well as different nitrogen sources ( peptone , yeast extract and urea ) on the production of B12 using Klebsilla pneumonia grown on mixture of agriculture wastes (wheat straw and rice bran ). The results showed that the priority of using glucose compared to the other di and polysaccharide used. The best B12 output (63.87ug/l) was obtained at 3glL glucose. On the other hand, peptone appeared to be the most suitable nitrogen source, since, it gave (57.8ug/l )of B12 compared to the other nitrogen sources used.Statistical analysis of the obtained results had been carried out and the data showed that glucose and peptone addition were highly significant compared to the other sources under investigation.

Keywords: Production, B12, solid state fermentation (SDF).


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