Abstract

CHEMICAL AND BIOLOGICAL CHARACTERIZE OF SOME SILENE SPECIES GROWING IN EGYPT

Ismail A Hussein, Abd-Elsalam I. Mohammad and Atef A. El-Hela*

Abstract

The present study aims to detect the phenolics and flavonoids constituents of three selected silene species belonging to caryophyllaceae family as well as screen their antioxidant and cytotoxic activities. These are Silene oliveriana Otth, Silene rubella L and Silene schimperiana Bioss. The phenolic and flavonoid profiles were carried out using colourmetric and HPLC technique. In addition, invetro antioxidant activity was performed using DPPH assay while the cytotoxicity was summarized by MTT test. Total phenolic and flavonoids (μg%) content in aerial parts of S. oliveriana, S. schimperiana and S. rubella were detected using colorimetric methods were found 3.7 , 15.2 ,7.2 and 32.09, 81.98, 50.70 respectively. Fourty compounds were detected using HPLC technique and comparison with standards. They were detected as 18 phenolic acids and 22 flavonoids. Ellagic acid was amajor content in phenolic acids (32.09, 81.98, 50.70 mg%) for S. oliveriana, S. rubella S schimperiana L respectively. The flavonoids were represented as eight- C-glycosyl flavones, two flavones, five flavonol glycosides, two flavones, four flavonol and two 2,3 dehydro flavone. Luteolin-6-arabinose-8- glucose was amajor componant of S. oliveriana(296.26), while Hesperdin was amajor componant of S. rubella and S schimperiana, (573.68 and 768.70 (μg %) respectively. The DPPH assay showed moderate activities with % activity 13.67±0.8, 45.23±0.34 and 23. 55± 0.8 and for S. oliveriana, S schimperiana, and S. rubella L respectively. Cytotoxicity of diffent and extracts have weak activity for the rest investigated plants on M-NFS-60, M-NFS- 60, MCF-7. HepG-2 cells, while n- hexane and ethylacetate extract of S.schimperiana showed significant cytotoxicity against colon carcinoma (HCT-116) and liver carcinoma(HPG-2) cells, with IC50 5.82,13.9 and 15.6, 20.5 respectively. Among the studied species, S,schimperiana showed the highest content of phenolic acids and flavonoids as well as a highly antioxidant and cytotoxic activities.

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