A BRIEF REVIEW ON ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY
Sathe Komal Pramod* and Khedkar Amol Navnath
Abstract
Ultra Performance Liquid Chromatography (UPLC) is a relatively new
technique. It is an analytical method commonly used in analytical
chemistry and in the pharmaceutical industry. Its operation is based on
column chromatography that is often used to investigate mixtures. This
technique gives new possibilities in liquid chromatography. The use of
which decreases the length of column, saves time and reduces solvent
consumption. It shows a great enhancement in speed, resolution and
sensitivity of analyses by using particle size less than 2μm. The quality
analyses of various pharmaceutical formulations are transferred from
HPLC to UPLC system. The separation on UPLC is performed under
very high pressure (up to 100MPa). Separation efficiency remains
maintained or is even improved by UPLC. This review introduces the
theory of UPLC.
Keywords: Chromatography, UPLC, Resolution, HPLC.
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