Abstract

IN VITRO EVALUATION OF METHANOLIC EXTRACT OF SCHEFFLERA VENULOSA FOR CYTO- COMPATIBILITY

Vikram R. Palled, Badarinath Kulkarni, Sudanshu Dubey and Harish K. Handral*

Abstract

The present workgives an insight of cytotoxic activity of the plant extract. Schefflera venulosa, which has been for its extensive use in the folk medicine. It is loaded with phyto-chemicals such as saponins, tannins, flavonoids, alkaloids, ardiac glycosides and reducing sugars. They bear the major activities like anti-inflammatory, anti-oxidant, Hepatoprotective. This present research work highlights the cytocompatible nature of S.venulosa methanolic extract on human cells. Objective:- To study the cyto-toxic effects of the methanolic leaf extract of S. venulosa. Methods:- The methanolic extraction of the plant leaf was obtained by standard methods. The Cytotoxicity of the plant extract was evaluated by performing MTT assay over MCF-7 (Epithelial Breast Cancer cell line) cell line. Result:- The methanolic extract showed cytotoxicity effect of S.venulosa on MCF-7 cell line by inhibiting MCF-7 growth/proliferation. The estimated methanolic extract showed moderate growth inhibition of MCF-7cells at 500μg/ml. Conclusion:- The study concludes, the leaf sample contains active compounds which are moderately cyto-toxic on the cancer cells, which was confirmed by the significant inhibition of cancer cell’s growth and killed them to certain extent in higher concentrations.

Keywords: Cytotoxicity, MCF-7, MTT assay, Schefflera venulosa, Methanolic Extract.