Abstract

FREE RADICAL SCAVENGING ACTIVITY OF ETHANOLIC EXTRACT OF BAUHINIA TOMENTOSA LEAVES COLLECTED FROM KOLLI HILLS (TAMIL NADU).

Aranganathan Jayaraman and Suriyavathana Muthukrishnan*

Abstract

The free radical scavenging potential of Bauhinia tomentosa (leaves) was assessed using varied in vitro antioxidant models. Reactive oxygen species (ROS), such as superoxide anions, hydrogen peroxide and hydroxyl, nitric oxide and peroxinitrite radicals, play an important role in oxidative stress related to the pathogenesis of various important diseases. Oxidation is essential to many living organisms for the production of energy to fuel biological process. Many oxidative stress related diseases are the result of accumulation of free radicals (in body).The % inhibition of ferric-reducing power against ascorbic acid and ferrous iron of Bauhinia tomentosa showed varied effect. It showed positive effect on reducing power. The scavenging effect of Bauhinia tomentosa seems to be higher, while the ability to scavenge the nitric oxide was noted to be lower. Among the radicals scavenging activity, Bauhinia tomentosa exhibited strong and effective activity against Hydrogen peroxide radical. The present study assessed the in vitro antioxidant activity such as 1,1-diphenyl-2-picryl hydrazyl, superoxide scavenging, reducing power, nitric oxide scavenging, 2,2 azino-bis (3- ethylbenzothiazoline-6-sulfonate), hydroxyl radical, ferric reducing antioxidant power, and chelating ability of ethanolic extract of Bauhinia tomentosa. Current research is directed towards finding naturally occurring antioxidants of plant origin. Scavenging ability of Bauhinia tomentosa against DPPH and ABTS was found to be effective compared with the standard( BHA). The antioxidative activity of the ethanolic extract of Bauhinia tomentosa was correlated with total phenolic content. Generally, the antioxidant activity of Bauhinia tomentosa ethanolic extract is comparable to a certain extent with that of butylated hydroxytoluene, mannitol, EDTA and ascorbic acid. The results from this study indicates that The free radical scavenging potential of Bauhinia tomentosa (leaves) was assessed using varied in vitro antioxidant models. Reactive oxygen species (ROS), such as superoxide anions, hydrogen peroxide and hydroxyl, nitric oxide and peroxinitrite radicals, play an important role in oxidative stress related to the pathogenesis of various important diseases. Oxidation is essential to many living organisms for the production of energy to fuel biological process. Many oxidative stress related diseases are the result of accumulation of free radicals (in body).The % inhibition of ferric-reducing power against ascorbic acid and ferrous iron of Bauhinia tomentosa showed varied effect. It showed positive effect on reducing power. The scavenging effect of Bauhinia tomentosa seems to be higher, while the ability to scavenge the nitric oxide was noted to be lower. Among the radicals scavenging activity, Bauhinia tomentosa exhibited strong and effective activity against Hydrogen peroxide radical. The present study assessed the in vitro antioxidant activity such as 1,1-diphenyl-2-picryl hydrazyl, superoxide scavenging, reducing power, nitric oxide scavenging, 2,2 azino-bis (3- ethylbenzothiazoline-6-sulfonate), hydroxyl radical, ferric reducing antioxidant power, and chelating ability of ethanolic extract of Bauhinia tomentosa. Current research is directed towards finding naturally occurring antioxidants of plant origin. Scavenging ability of Bauhinia tomentosa against DPPH and ABTS was found to be effective compared with the standard( BHA). The antioxidative activity of the ethanolic extract of Bauhinia tomentosa was correlated with total phenolic content. Generally, the antioxidant activity of Bauhinia tomentosa ethanolic extract is comparable to a certain extent with that of butylated hydroxytoluene, mannitol, EDTA and ascorbic acid. The results from this study indicates that

Keywords: BHA, Bauhinia tomentosa, DPPH, FRAP, In vitro antioxidants.