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Abstract

SENSITIVE AND VALIDATED U.V.SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF NIMESULIDE IN PHARMACEUTICAL AND BULK FORMULATIONS

N.Mamatha*, K.Radhika, A Santosh kumar Sreevatsav

Abstract

Two simple precise and accurate U.V Spectrophotometric methods were developed and validated according to ICH guidelines. Method A: methanol was used as a solvent and absorption maximum was found at 297nm.method B: methanol: acetonitrile (50:50) was used as a solvent and absorption maximum was found at 295nm.Validation parameters such as accuracy, linearity, robustness were performed. % assay and %RSD was calculated and results were found within the predetermined limits. This indicated that the proposed methods can be used in routine analysis of Nimesulide in bulk and pharmaceutical formulations.

Keywords: U.V., RSD, acetonitrile


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