Abstract

ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF CLITORIA TERNATEA LINN. AGAINST PROTEUS MIRABILIS FROM URINARY TRACT INFECTED PATIENTS

Dr. K V Radha , Dr.T.Arun and *Srinivas Pidugu

Abstract

The disease preventive and health promoting approach based on herbal medicine takes into consideration, the promotion of health and treating ailments is holistic way and finds increasing acceptability in many regions of the world, India and China being in the forefront. The practice of herbal medicine basically depends on folklore and by experiences of people over a long period of time. However, very little research especially in the area of basic science has been conducted in a systematic manner. Medicinal plants with a long history of safe and effective use are likely to have a pharmaceutical effect. The use of plants in traditional medicine can be explained by physiologically active phytochemical compounds of a species and also by its ascribed meaning in a culture. Plants produce a great diversity of substances that could be of therapeutic significance in many areas of medicine. Several aspects on integrated approaches of drug development from herbs have explored many potential lead phytochemicals. In the present study 63 urine samples were collected from Urinary tract infected patients from various hospitals in Salem District, Tamilnadu, India. The collected samples were subjected to microscopic observation and biochemical characterization to identify the presence of bacteria The Proteus mirabilis were isolated on specific medium using XLD (xylose lysine deoxycholates agar deficient ) , Macconkey agar , Mullen hinton agar, CLED and UTI agar. The purified Proteus mirabilis were performed in biochemical tests such as IMVIC tests etc. The positive isolate was used for antibacterial activity for Leaves of Clitoria ternatea Linn. were extracted by using acetone, isopropyl alcohol and petroleum ether, the compound was purified and preliminary phytocemical studies was done. A comparative study on the total antibiotic activity of plant extract were found to be effective against the tested isolated organism Proteus mirabilis and MTCC 442 strain. MIC and MBC was performed by agar dilution method and the range was found to be 0.97mg/ml to 250 mg/ml. from the result that particular plant extract (Clitoria ternatea Linn.) showed high antibacterial activity against tested organism.

Keywords: UTI infected urine samples, Proteus mirabilis, Antibacterial activity, MIC and MBC, Physical constants of Clitoria ternatea Linn, Phytochemical screening.