Abstract

SYNTHESIS OF O-(4-FLUOROBENZOYL) ACETAMINOPHEN AND ANALGESIC ACTIVITY TEST IN MICE (MUS MUSCULUS)

Ulfa Fikria Putri*, Dwita Saraswati, Naili Uswatun Hasanah, Bambang Tri Purwanto, Siswandono

Abstract

Acetaminophen is an analgesic drug that is often used. Acetaminophen can cause hepatotoxicity if it is consumed for a long period of time or consumed in high dose. Hepatotoxicity is caused by the formation of acetaminophen’s metabolite (NAPQI) which bind the liver cell at the ortho position of the hydroxyl group. The purpose of this study is to synthesize acetaminophen derivate O-(4-fluorobenzoyl)acetaminophen and evaluate its analgesic activity. Synthesis O-(4- fluorobenzoyl)acetaminophen was carried out by reacting acetaminophen with 4-fluorobenzoyl chloride. The TLC and melting point test indicated the purity of the compound. Structure identification was done based on UV-Vis, FT-IR, and 1H-NMR spectra. The synthesized compound was tested for analgesic activity by hot plate method in mice (Mus musculus) and the result showed that O-(4-fluorobenzoyl)acetaminophen has higher analgesic activity than acetaminophen.

Keywords: Synthesis; O-(4-fluorobenzoyl) Acetaminophen, Analgesic Activity.