Abstract

METHOD VALIDATION AND SIMULTANEOUS DETERMINATION OF TWO BIO-ACTIVE MARKER COMPOUNDS IN PONGAMIA PINNATA: SEED AND KARANJ OIL BY UPLC-MS

N.M.A. Rasheed, K. Nagaiah*, G. Sowmya Srividya and M.V.N. Kumar Talluri

Abstract

Seed of Pongamia pinnata (Linn.) Merr known as "Karanj" widely used in traditional system of medicine. Oil from karanj seeds contain flavonoids which possess diverse pharmacological properties. The seed oil was subjected to karanj oil-liquid extraction with methanol to isolate karanjin and pongamol which are active principle of karanj. The oil mainly used for skin diseases such as psoriasis, eczema and vitiligo etc. A validated reversed-phase ultra-performance liquid chromatography method with photodiode array detector was successfully developed for the first time report to simultaneously determine two active compounds karanjin and pongamol in karanj seed extract and its oil. Separation carried through a BEH C18 column by gradient elution using 0.1% formic acid in water (v/v): methanol: acetonitrile at 0.2 mL/min. Significant linear correlations were found between component concentrations and specific chromatographic peak areas. The % R.S.D. values found by the method indicating precision, range and recovery to be good. Thus the proposed validated method can be applied as a reference standard for quality control analysis commercial samples of karanj oil.

Keywords: Karanj oil, Pongamia Pinnata, UPLC, Karanjin, Pongamol.