Abstract

FORMULATION AND EVALUATION OF MICROSPHERES CONTATING LAMAVUDINE

Deepa Kantekar*, Ajay Kumar D., B. Prashant and R. Anusha naik

Abstract

Lamivudine is an antiretroviral drug, specifically a nucleoside reverse transcriptase inhibitor. It is used to treat HIV, a retrovirus. Retroviruses use the genetic material in the body’s cells to produce more viruses which can infect other cells. Adverse effect of lamuvidine headache, fever, chills, and muscle aches, dizziness, nausea, vomiting, insomnia, restlessness, and rash. The main objective of this research work was to prepare hydroxypropyl methyl cellulose microspheres loaded with lamuvidine and Invitro release study. In the present study, emulsification heat stabilization method is used in the preparation microspheres. The polymer hydroxyproply methylcellulose was dissolved and added to the solution and stirred it for 2 hrs. In this experiment, lamuvidine 100mg was taken and dissolved in span-60 10-15ml. then 100mg hydroxypropyl methylcellulose is taken and dissolve in 10-15ml. these both beakers are kept aside until the solutions get dissolved. Then these 2 dissolution are taken in one beaker. The drug is added in the polymer solution drop by drop with the help of mechanical stirrer resulting in formation of microspheres. Microspheres were spherical shape and smooth surface. Infrared spectra showed identical peaks of the drug and polymer. Drug entrapment efficiency was determined by uv-spectrophotometer at 254 nm. In vitro release studies were performed by using shaking flask method about drug was released in 6hrs. It is concluded that hydroxlyproply methylcellulose and microspheres of lamuvidine can be prepared by emulsification heat stabilization in vitro release data is satisfactory.

Keywords: Lamivudine, span-60, hydroxyproply methylcellulose, microspheres.