Abstract

GSTM1 NULL GENOTYPE ASSOCIATED WITH TYPE 2 DIABETIC NEPHROPATHY PATIENTS AMONG INDIAN POPULATION

Pulakes Purkait; Kalpataru Halder; Abhishikta Ghosh Roy; B.N. Sarkar*;J.M. Naidu

Abstract

Oxidative stress is an imperative factor in the etiology and pathogenesis of diabetes. Glutathione S transferases are multifunctional enzymes, which act as a kind of antioxidant defense. The GSTM1null allele represents a deletion of the GSTM1 and result in a loss of enzymatic activity. Previous studies reported that GSTM1 null genotype is associated with type 2 diabetes and diabetic nephropathy in different populations. The aim of this study is to investigate the association of GSTM1 gene polymorphism in the type 2 diabetes mellitus patients with and without nephropathy. For the present study 356 age matched participants (123 T2DM, 84 T2DN patients and 152 healthy controls) belonging to Bengali population from Kolkata city were enrolled and 10ml of peripheral blood sample was collected from each individual. Genomic DNA was prepared from fresh whole blood by using the conventional method and polymerase chain reaction was carried out to identify the null genotype of GSTM1 gene. Data were analyzed using statistical software SPSS 16 and MINITAB 11. The present study reveals that anthropometric measurements and bio-chemical parameters were significantly varying in three groups, i.e. T2DM, T2DN and control. Distributions of GSTM1 null genotypes was 24 (19.51%) in the T2DM patients, 31 (36.90 %) in the T2DN patients and 20 (13.16 %) in the controls. The frequency of GSTM1 null genotype was significantly increased in the diabetic nephropathy patients. Our results suggest that GSTM1 gene polymorphisms may play an important role in pathogenesis of type 2 diabetes mellitus with and without nephropathy patients among the Indian Bengali population.

Keywords: Type 2 diabetes mellitus, Type 2 diabetic nephropathy, GSTM1, Null genotype, Indian population.