Abstract

MOLECULAR IDENTIFICATION AND CHARACTERIZATION OF BACTERIAL SPECIES WITH POTENTIAL SCREENING ACTIVITY FROM A MARINE COASTAL ENVIRONMENT

*P. N. Rajarajan, P. Jeganathan, K. Rajeswari and N. Sumathy

Abstract

Marine seawater samples were collected from Rameswaram, Tamilnadu. Six bacteria were isolated and named MB1, MB2, MB3, MB4, MB5, and MB6. All the six isolates were identified according to morphology, biochemical tests and selective growth media. The isolates were tested for antagonistic activity against Salmonella typhi, Enterobacter sp. and Pseudomonas sp. Only 2 isolates of MB2 and MB5 showed clean growth inhibition zone against salmonella typhi, Enterobacter sp. and Pseudomonas sp. From the selected isolates of MB2 and MB5 were grown on Nutrient broth and mature broth were centrifuged at 1500 rpm for 15 minutes. The supernatant layer was collected and which was used for compound isolation studies by NMR. Two compounds of Dreschsterin - A and Cis-Sativenediol were identified in MB2 and MB5 respectively based on NMR spectra. It can be concluded that isolation of Marine bacterial samples can offer a numbers of microbial strains for sources of new biomolecules from Marine sources. Bacteria are known to produce bioactive substances in the Marine environment of they are specifically antibiotic principles. This study indicated that certain marine bacteria could produce potential biomolecules for treat varieties of human diseases.

Keywords: Marine bacterial isolates, NMR study, Dreschsterin - A, Cis - Sativenediol.