Abstract

IMPACT OF GANODERMA LUCIDIUM ON CARBON TETRA CHLORIDE (CCl4) INDUCED HAEMATOLOGICAL PROFILES OF MICE

*Mohammad Ali, Shikha, S. Shamim Ahmed, R. Kumar, J.K. Singh, & A. Kumar

Abstract

Aim : To investigate the impact of Ganoderma lucidium on carbon tetrachloride (CCl4) induced haematological profiles of swiss albino mice. Methods : Thirty mice were used in the study and were grouped into three with 10 mice in each group. Group A: untreated mice kept as control and served with equal volume of distilled water by gavage method. Group B: mice treated with 2.8 ml/kg b. w. of (CCl4) twice a week for 5 weeks to make anemic & immunosuppressant model. Group C: mice treated with Ganoderma lucidium followed by CCl4 by gavage method @ 75 mg/kg b.w. (body weight) for eight weeks. After making several trials of dose of Ganoderma lucidium, oral dose of 75 mg/kg b.w. (body weight) was used for amelioration. The treated and control group of mice were sacrificed on targeted day for haematological study. Haematological parameters such as RBC count, Total WBC count, PCV, Hb (%), and platelets count were determined. Results : There was significant statistical difference (p < 0.001) was observed in the PCV of CCL4 treated group with compare to control. A significant increase however was seen in the G. lucidium treated group of PCV. There was also significant statistical difference (p < 0.001) were observed in the red blood cells (RBC), haemoglobin, white blood cells (WBC) & platelets (PLT) with compare to control. A marked significant statistical increase (p < 0.001) was observed in the RBC, WBC, & Hb during the period of the study in the G. lucidium treated group except platelets count. There was no significant statistical difference (p> 0.05) in the platelets count was seen in all groups during the period of the study. Conclusion: Oral administration of G. lucidium significantly reduces CCl4-induced haematological alterations in mice, probably by exerting a protective effect by its free-radical scavenging and immunomodulator ability.

Keywords: Ganoderma lucidium, CCl4 , swiss albino mice, haematological profiles.