STUDY OF BIOACTIVE COMPONENTS OF N-BUTANOL BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY (GC-MS) IN LEAVES OF GREWIA TENAX GROWING IN KACHCHH DISTRICT
*Vijay R. Ram, Mital K. Aadesariya and Suhas J. Vyas
Abstract
Grewia tenax (GT) is the mutual name of Guddaim or Gangeti, a
treasured plant species in Kachchh region.Leaves of G. tenax are a
significant part of the traditional remedy for the use of treatment
against tonsillitis infections, trachoma and are used as a compress
against inflammation. Grewia’s extracts are also useful in remedial
hepatitis and another such type of diseases. Plant containshigh
therapeutic standards. It has been used to treatseveral diseases such as
dysentery, fever, rheumatism, diarrhea, anemia, osteoporosis, body
weakness nausea, bone fractures, bone strengthening etc. Present study
has been carried out on thequalitative and quantitative analysis of the major bioactive
components of therapeutically significant plant Grewia tenax (GT) leaves by the use of GCMS,
whereas the mass fragment spectra of the compounds was compare with the National
Institute of Standards and Technology(NIST) library. The soxhlet extraction of sample was
done by use of continuous hot percolation method using n-Butanol as a solvent. After
extraction, it was concentrated by using distillation method. Crude n-Butanol extracts were
introduced in GC/MS instrument for isolation and identification of valuable phytochemicals.
GC-MS analysis hasrevealed the existence of 27 compounds.The result exhibited that there
are very significant phytochemicals found in n-Butanol leaves extract of G. tenaxlikeFatty
acid, Hydrocarbons, Carbohydrate, Diterpenoid, Diterpenes, Triterpen, Phytosterol, Vitamin
E and Steroid compounds.
Keywords: Grewia tenax, GC/MS, Phytochemicals, n-Butanol extract, Soxhlet extraction.
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