Abstract

STUDY OF BIOACTIVE COMPONENTS OF N-BUTANOL BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY (GC-MS) IN LEAVES OF GREWIA TENAX GROWING IN KACHCHH DISTRICT

*Vijay R. Ram, Mital K. Aadesariya and Suhas J. Vyas

Abstract

Grewia tenax (GT) is the mutual name of Guddaim or Gangeti, a treasured plant species in Kachchh region.Leaves of G. tenax are a significant part of the traditional remedy for the use of treatment against tonsillitis infections, trachoma and are used as a compress against inflammation. Grewia’s extracts are also useful in remedial hepatitis and another such type of diseases. Plant containshigh therapeutic standards. It has been used to treatseveral diseases such as dysentery, fever, rheumatism, diarrhea, anemia, osteoporosis, body weakness nausea, bone fractures, bone strengthening etc. Present study has been carried out on thequalitative and quantitative analysis of the major bioactive components of therapeutically significant plant Grewia tenax (GT) leaves by the use of GCMS, whereas the mass fragment spectra of the compounds was compare with the National Institute of Standards and Technology(NIST) library. The soxhlet extraction of sample was done by use of continuous hot percolation method using n-Butanol as a solvent. After extraction, it was concentrated by using distillation method. Crude n-Butanol extracts were introduced in GC/MS instrument for isolation and identification of valuable phytochemicals. GC-MS analysis hasrevealed the existence of 27 compounds.The result exhibited that there are very significant phytochemicals found in n-Butanol leaves extract of G. tenaxlikeFatty acid, Hydrocarbons, Carbohydrate, Diterpenoid, Diterpenes, Triterpen, Phytosterol, Vitamin E and Steroid compounds.

Keywords: Grewia tenax, GC/MS, Phytochemicals, n-Butanol extract, Soxhlet extraction.