FORMULATION AND EVALUATION OF NANOPARTICLES CONTAINING CYCLOPHOSPHAMIDE
*Pankaj Sharma, Sarvesh Bhargava, Deepesh Parashar and Ashish Mangal
Abstract
Objectives of present study were- to maintain the therapeutic drug
concentration in the site of action for a prolonged period, improve the
drug’s efficiency & reduce the dose related side effect of
cyclophosphamide. Cyclophosphamide nanoparticles were prepared by
salting out method. Nine (F1-F9) formulations of nanoparticles were
developed in research. After development of formulations, various
evaluation parameters were calculated. Percentage yield of
nanoparticles were determined for all 9 formulations (F1 to F9). The
result for all different formulated was obtained in the range of
26.09±0.95 to 39.8±1.4. Loading efficiency was determined for all
nine formulations (F1 to F9). The results for all different formulations were obtained in the
range from 40.56±0.85 to 45.29±2.1. Particle size determination was done by using Malvern
mastersizer instrument for the optimized formulation F8. Particle size was found 730.9 nm
with maximum intensity and volume. From this data, it could be said that the nanoparticles
has been formed successfully by using ionic cross linking method. Polydispersibility index of
the cyclophosphamide obtained nanoparticles was found to be 0.918 and hence shows a
narrow size distribution and contributes to the stability of nanoparticles. Based on the
observations, formulation F8can be concluded that the PLGA coated Nano particulate
targeted delivery system of cyclophosphamide formulated using widely accepted and
physiologically safe polymer was capable of exhibiting sustained release properties for 8hrs.
Keywords: Nanoparticles, FTIR, Polydispersibility, Entrapment Efficiency.
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