Abstract

PHYTOCHEMICAL ANALYSIS OF THE METHANOLIC EXTRACT OF GRACILARIA TEXTORII (SURING.) J. AG. FROM KANYAKUMARI IN THE SOUTH EAST COAST OF TAMIL NADU, INDIA

Iniya Udhaya C. and John Peter Paul J.*

Abstract

The objective of the present study was to screen the phytoconstituents composition of methanol extract of Gracilaria textorii (Suring.) J. Ag. collected from Kanyakumari, in the south east coast of Tamil Nadu, India. The phytochemical screening of methanol extract was estimated using the standard procedure for UV-Vis spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed five compounds separated at the wavelength of 662nm, 606nm, 536nm, 503.5nm and 400nm with the absorption 0.608, 0.182, 0.213, 0.287 and 2.289 respectively. The qualitative HPLC fingerprint profile displayed seventeen compounds at different retention times. The profile displayed seven prominent peaks at the retention time of 1.717min, 2.217min, 2.313min, 2.777min, 3.120min, 7.610min and 11.510min, followed by ten moderate peaks were observed at the retention time of 2.057min, 2.623min, 2.963min, 3.730min, 3.987min, 4.413min, 4.840min, 5.473min, 6.380min and 9.587min. The FTIR analysis recognized different peaks at 526.53, 745.44, 1040.52, 1040.52, 1205.43, 1404.08, 1660.60, 2850.59, 2920.03, 3380.98 and 3443.66cm-1 with the presence of functional groups such as naphthalenes, disubst benzenes, organophosphorus, vinyl ethers, primary amides, primary amides, aliphatic, aliphatic, alcohols, phenols, aromatic, primary amines and amides.

Keywords: Gracilaria textorii, Red seaweed, Phytochemical, UV-Vis, HPLC, FTIR.