PHYTOCHEMICAL ANALYSIS OF THE METHANOLIC EXTRACT OF GRACILARIA TEXTORII (SURING.) J. AG. FROM KANYAKUMARI IN THE SOUTH EAST COAST OF TAMIL NADU, INDIA
Iniya Udhaya C. and John Peter Paul J.*
Abstract
The objective of the present study was to screen the phytoconstituents
composition of methanol extract of Gracilaria textorii (Suring.) J. Ag.
collected from Kanyakumari, in the south east coast of Tamil Nadu,
India. The phytochemical screening of methanol extract was estimated
using the standard procedure for UV-Vis spectroscopic, HPLC and
FTIR. The UV-Visible spectrum showed five compounds separated at
the wavelength of 662nm, 606nm, 536nm, 503.5nm and 400nm with
the absorption 0.608, 0.182, 0.213, 0.287 and 2.289 respectively. The
qualitative HPLC fingerprint profile displayed seventeen compounds at
different retention times. The profile displayed seven prominent peaks
at the retention time of 1.717min, 2.217min, 2.313min, 2.777min,
3.120min, 7.610min and 11.510min, followed by ten moderate peaks
were observed at the retention time of 2.057min, 2.623min, 2.963min,
3.730min, 3.987min, 4.413min, 4.840min, 5.473min, 6.380min and
9.587min. The FTIR analysis recognized different peaks at 526.53,
745.44, 1040.52, 1040.52, 1205.43, 1404.08, 1660.60, 2850.59,
2920.03, 3380.98 and 3443.66cm-1 with the presence of functional groups such as
naphthalenes, disubst benzenes, organophosphorus, vinyl ethers, primary amides, primary
amides, aliphatic, aliphatic, alcohols, phenols, aromatic, primary amines and amides.
Keywords: Gracilaria textorii, Red seaweed, Phytochemical, UV-Vis, HPLC, FTIR.
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