Abstract

A STUDY ON THERAPEUTIC EFFECTS OF ULTRASOUND FOR THE TREATMENT OF SARCOMA CANCER

C. S. Ram, R. K. Saxena, Jayanand, Sonali Gangwar, Maya Datt Joshi and D.V. Rai*

Abstract

Context: Ultrasound is emerging as a novel treatment agent for cancer. The advantage of using ultrasound is that it is not an electromagnetic radiation; hence it does not produce the undesired harmful effects encountered through the repeated use of electromagnetic radiation. Aims: The present study was aimed to evaluate the therapeutic potential of ultrasound in 7, 12-dimethyl benz(a)anthracene (DMBA) induced sarcoma in rats. Settings and Design: Forty female wistar rats were used in the experimental study. They were allocated in four groups.7, 12-dimethyl benz(a)anthracene (DMBA) was used to induce sarcoma in 20 rats. Therapeutic ultrasound was applied at 2.6W/cm2 for 10 min (continuous mode) to 10 sarcoma tumor bearing rats and normal 10 rats. Materials and Methods: 7, 12- dimethyl benz(a)anthracene (DMBA) was used to induced sarcoma in rats. Body weight, Tumor weight, Serum enzymes were determined following treatment with therapeutic ultrasound (Chattanooga Corp Inc. USA). Statistical analysis used: Statistical analysis was performed using SPSS (SPSS Inc., Chicago) statistical package. The results were expressed as Mean, Standard Error Mean (SEM). One-way analysis of variance (ANOVA) followed by post hoc test least significant difference (LSD) was used to correlate the difference between the variables. Results: There were significant increases on the body weight and tumor weight of treated rats. The increased activities of serum pathophysiological enzymes AST, ALT, ALP, ACP, and LDH of ultrasound treated rats were significantly (p < 0.05) higher than control levels indicating loss of redox homeostasis. The histopathological analysis of sarcoma tissues showed extensive haemorrhage and necrosis indicating the anti-tumor nature of ultrasound. Conclusions: The results of the present study indicate that ultrasound significantly suppresses DMBA induced sarcoma in rats.

Keywords: Therapeutic, Ultrasound, Sarcoma, Cancer.