Abstract

GREEN SYNTHESIS OF GOLD NANOPARTICLES USING LEAF EXTRACT OF GARCINIA COMBOGIA, ITS GENOTOXIC EFFECTS ON E.COLI DNA AND CYTOTOXIC EFFECTS ON HE LA CELL LINES.

B. Nithya* and A. Jayachitra

Abstract

Cancer is a mortiferous disease or a destructive phenomenon emergesin human beings as a result of genetic factors or disclosure to harmfulchemicals or viral infections etc. Compared to other treatments Radiotherapy is potential in the treatment of cancer. In this therapy, despitethe normal cells are also disintegrated in the fore with tumor cells.Nanomedicine is a relavent treatment of cancer is an innovativelanding. Progressing consignment of anticancer agents to tumors usingnanoparticles is one of the most protrusive combat zone in the researchfield of nanotechnology. It can be known from research that MTTassay is broadly applied and extensively approved method in findinginvitro anticancer activity of metal nanoparticles. Gold nanoparticles biosynthesis isupsurging from day to day generating an upshot in all biological applications. Greensynthesized gold nanoparticle inhibit the proliferation of cancer cells by activating theapoptosis process or changing the cellular chemistry. Present investigation reveal the goldnanoparticles biosynthesizing capability of the leaf of Garcinia combogia. Newly genre goldnanoparticles were characterized by involving UV–vis spectroscopy, FTIR, XRD, SEM,analysis. Strikingly as a result of extensive screening on the application of newly synthesizedgold nanoparticles their anticancer cytotoxicity has been discovered using MTT assay and itsgenotoxicity by DNA fragmentation.

Keywords: Garcinia combogia, Gold nanoparticles, MTTassay, DNA fragmentation.