INHIBITION OF PANCREATIC LIPASE ACTIVITY BY PLUMERIA PUDICA LEAVES; AN APPROACH TO THE TREATMENT OF OBESITY
Vyshali V. M.* and Shuba S. V.
Abstract
Obesity is usually measured as an overweight or surplus body fat,
leading to increased health problems; it is a major peril factor for a
number of serious diseases. Decreasing dietary fat absorption, through
inhibition of pancreatic lipase activity, has been reported to be one of
the most effective ways for controlling obesity. The present study was
aimed at investigating lipase inhibitors from plumeria pudica leaves.
The powdered leaves material was extracted with methanol in soxhlet
apparatus. Compounds were analysed by Gass chromatography and
mass spectroscopy. Lipase enzyme was obtained from the chicken
pancreas. Lipase inhibitory activity of different concentrations of methanol extract was
determined in terms of inhibition of lipase activity using olive oil as substrate. The extract
was found to inhibit activity of lipase and the effect was found to be concentration dependent.
Therefore, plumeria pudica leaves may be further developed to use for weight management
and isolation for the active compounds.
Keywords: Lipase enzyme, Gass chromatography and mass spectroscopy.
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