Abstract

INHIBITION OF PANCREATIC LIPASE ACTIVITY BY PLUMERIA PUDICA LEAVES; AN APPROACH TO THE TREATMENT OF OBESITY

Vyshali V. M.* and Shuba S. V.

Abstract

Obesity is usually measured as an overweight or surplus body fat, leading to increased health problems; it is a major peril factor for a number of serious diseases. Decreasing dietary fat absorption, through inhibition of pancreatic lipase activity, has been reported to be one of the most effective ways for controlling obesity. The present study was aimed at investigating lipase inhibitors from plumeria pudica leaves. The powdered leaves material was extracted with methanol in soxhlet apparatus. Compounds were analysed by Gass chromatography and mass spectroscopy. Lipase enzyme was obtained from the chicken pancreas. Lipase inhibitory activity of different concentrations of methanol extract was determined in terms of inhibition of lipase activity using olive oil as substrate. The extract was found to inhibit activity of lipase and the effect was found to be concentration dependent. Therefore, plumeria pudica leaves may be further developed to use for weight management and isolation for the active compounds.

Keywords: Lipase enzyme, Gass chromatography and mass spectroscopy.