Abstract

DEVELOPMENT OF HERBAL FORMULATION WITH AJWA SEED (PHOENIX DACTYLIFERA L.) EXTRACT AND ZAM-ZAM WATER FOR INVITRO-ANTICANCER ACTIVITY

Ibrahim Afsal V. T.* and B. Arul

Abstract

Objective: Fruits of the date palm (Phoenix dactylifera L.) Belonging to the family of Arecaceae are very commonly consumed in many parts of the world and are a vital component of the diet in most of the Arabian countries. One of the miracles of Zamzam water has been collected by scientists and they have found certain peculiarities that make the water healthier, like a higher level of calcium. Hence, the present study was intended to evaluate ethanolic seed (Fruits of the date palm) extract of Phoenix dactylifera L for anticancer activities. Materials and Methods: In the present study investigated on different cancer cell lines such as HL-60, HT-29, A 549, A 431 and MCF-7. The activity was assessed by trypan blue dye exclusion assay for cell viability, MTT and SRB based cytotoxicity assays. Results: Results indicate that the anti proliferative effect strengthens with increase in the concentration of ethanolic seeds extract of Phoenix dactylifera L. Highest cytotoxicity was found against HL-60 and HT-29. However ethanolic extract have moderate activity on A 549, A 431, and MCF-7. Effect on Hep 2 cell line upto 0.0196 mg/ml and that IC50 value on Hep 2 cell line were 468; Phoenix dactylifera L has no effect on normal healthy body cell So Phoenix dactylifera L second hand as antitumour action to diminish the side effect. Conclusion: It can be concluded that the anticancer activity elucidated by Phoenix dactylifera L could be mainly due to the presences of high value class of compound like phenolic group as the major content in the seed extract.

Keywords: Phoenix dactylifera L, Zamzam water, cell viability, MTT, SRB, Trypan blue dye, cyclophosphamide.