Abstract

ANTI-ARTHRITIC AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF RANDIA DUMETORUM FRUITS IN FREUND’S COMPLETE ADJUVANT INDUCED ARTHRITIS

Abstract

Randia dumetorum has multiple applications in traditional medicine because it exhibit Analgesic, Antifertility, Antibacterial, Antiinflammatory, Antiulcer, and Protective effects. However, no study on the anti-arthritic activity of Randia dumetorum has been reported in vivo. The present study was undertaken to determine efficacy of madecassoside (MA) against Freund’s complete adjuvant-induced arthritis (CFA) in female rat. Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic inflammation. It is characterized by hyperplasia of synovial cells in affected joints, which ultimately leads to the destruction of cartilage and bone. We investigated therapeutic efficacy of Randia dumetorum in treating Rheumatoid Arthritis (RA) using Freund’s complete adjuvant-induced arthritis (CFA) animal model. Arthritis was induced in female Wistar rats by intradermal injection of Freund’s complete adjuvant (CFA). CFA rats were treated daily with oral administration of different doses of Methanolic extract of Randia dumetorum(RD), beginning on the day after the onset of arthritis (day 21st, the therapeutic treatment) until day 28th. The results showed that treatment with Randia dumetorum markedly reduced paw swelling and arthritic index even in the established CFA. Radiologic and histopathologic changes in the arthritic joints were also significantly reduced in the Randia dumetorum -treated versus vehicle-treated rats. Moreover, Rheumatoid factor was significantly reduced in Randia dumetorum treated group in compared to disease control group. Hence, our studies demonstrate safety, and effectiveness of Randia dumetorum as an anti-arthritic agent, which makes Randia dumetorum a strong candidate for further research on rheumatoid arthritis (RA).

Keywords: Randia dumetorum, Rheumatoid arthritis, Freund’s complete adjuvant-induced arthritis.