Abstract

FREE RADICAL SCAVENGING ACTIVITY OF PREMNA INTEGRIFOLIA LINN.

*Chitra Subramani, Arunadevi Rathinam, Ilavarasan Raju, Sudesh Gaidhani

Abstract

Purpose: The present paper was aimed to study an in-vitro and in-vivo antioxidant activities of hydro alcoholic extracts (HAE) of root bark of P. integrifolia. Methods: The hydro alcoholic extract was subjected to various in-vitro assays such as DPPH, nitric oxide scavenging activity, reducing power assay, superoxide anion radical scavenging and iron chelation assays. For in-vivo analysis sixty Wistar rats were taken and they were divided into six groups; the first group served as control, the second group was fed with high fat diet, the other three groups were fed with high fat diet along with various concentrations of HAE and the last group was treated with atorvastatin for 30 days. Analysis of lipid peroxides, superoxide dismutase (SOD) and catalase activities were carried out. Results: The IC50 values of 142.93, 293.9, 1000, 810.67, 69.03μg/ml for DPPH, nitric oxide, reducing power assay, superoxide anion radical scavenging and iron chelation assays respectively were found in the present study. The lipid peroxides and conjugated dienes were increased and SOD, catalase activities were decreased in blood, liver, heart and kidney of atherosclerosis induced rats when compared to control. The activities were reversed to near normalcy on treatment with different doses of HAE. Conclusion: HAE extract was showed an increased activity by 3.21 fold and 3.09 fold in nitric oxide and DPPH scavenging activity respectively. The activity was directly proportional to the concentration of the HAE treatment. The in-vitro free radical scavenging activity is correlated well with the in-vivo anti oxidant activities in high fat diet induced atherosclerosis.

Keywords: Nitric oxide; DPPH; SOD; Lipid peroxides; Premna integrifolia.