Abstract

PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF PERGULARIA DAEMIA (FORSK) CHIOV, AGAINST MULTIDRUG RESISTANCE HUMAN PATHOGENIC BACTERIA

Madhuri M. Deshmukh* and Navnath G. Kashid

Abstract

The increasing number of multidrug resistant bacteria to conventional antibiotics has become a serious problem in recent years. So there is alarming need to investigate new antibiotics and alternative products to solve this problem. This research aim is to search potent herbal drugs from Pergularia daemia extracted in different solvent system considering their polarity and non polarity nature. Pergularia daemia stem part extracted in different solvents, Methanol, Ethanol, Chloroform, Petroleum Ether, Ethyl Acetate and Aqueous solution. The technique used for extraction is of Soxhlet Apparatus and Rotary evaporator. Effective extracts obtain tested against some pathogenic bacteria, Escherichia coli, (ATCC-25922), staphylococcus aureus (25923ATCC) Klebsiella pneumonia, Salmonella typhi (734 MTCC). Among all tested extracts aqueous extract proved most powerful antibacterial activity compare to standard antibiotics amoxicillin for these bacteria, aqueous followed by ethyl acetate. Methanol and Ethanol extract showed less antibacterial effect. Chloroform and Petroleum Ether extract does not showed any activity. Antibacterial activity of solvent extracts was tested by Disc Diffusion and Agar Well Diffusion method. This research also highlights on the existing information particularly on the phytochemistry alkaloid, phenolic compound, steroids, flavonoids, which may provide incentive for proper evaluation of the plant as a medicinal agent.

Keywords: Pergularia daemia, Antimicrobial activity, Human Pathogen, E.coli, S. aureus, Kleb. Pneumoniae, S. typhi. secondary metabolites.