WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

HP-TLC DENSITOMETRIC ANALYSIS OF LUPEOL FROM ELEPHANTOPUS SCABER [LINN.]

Ashwini Surendra Shinde*

Abstract

A high performance thin layer chromatography (HP-TLC) method was developed for identification, quantification and validation of lupeol from methanolic and hydro-methanolic extract of Elephantopus scaber L. TLC Si gel 60 F254 precoated plate was used as a stationary phase. Toluene: Ethyl acetate: Formic acid (12:7:1 v/v/v) was used as a mobile phase for better separation and resolution. Quantification was performed by densitometric scanning at 540 nm after derivatizating with anisaldehyde sulphuric acid reagent (ASR). Compact spot at Rf 0.67 was obtained corresponding to standard lupeol in the plant sample. According to the ICH guidelines, this method was validated in terms of specificity, precision and accuracy assay. Linearity range for lupeol was 100 – 800 ng/band with correlation of coefficient (r2) = 0.99319. The LOD and LOQ were found to be 9.34 and 28.31 ng/band, respectively. The lupeol content in methanol and hydromethanol extract were found to be 386.0 and 301.3 μg/mg, respectively. The HP-TLC method was found to be rapid, simple, specific, precise, accurate and convenient method for screening of active principles present in the plant extracts. This method can also be used for routine analysis of quality control of herbal formulations containing E. scaber.

Keywords: Elephantopus scaber L., HP-TLC, Lupeol, Method development, Quantification, Validation.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More