Abstract

CALCIUM PHOSPHATE NANOCOMPOSITE FOR RAPID FLUOROGENIC DETECTION OF PATHOGEN

Devipriya R. Majumder*, Juned Pathan and Hemant Zimbar

Abstract

This study was adopted to decipher the detection method of bacterial pathogens by Tri-Calcium phosphate nanocomposite. The conventional process for detection of bacterial infections is time-consuming, and expensive. Therefore in this research, a simple, fast, and low cost method for bacterial detection has been developed, based on calcium phosphate nanoparticle with a nutritive mixture and the fluorogenic substrate 4-methylumbellipheryl-β-D-glucuronide (MUG).The fungal species have been known to show the property of synthesizing phosphorous nanoparticles. Hence the fungus, Aspergillus sp. was exploited for nanoparticle synthesis. The fungal mycelium were grown to form balls of mycelium which were then extracted to synthesize calcium phosphate nanoparticles by using salt solution of 0.01 M Tri- Calcium phosphate. Then, these nanoparticles were reacted with high rapid coliform agar which promotes quick multiplication of bacteria and MUG, which acts as a flurogenic substrate in synthesis of nanocomposite. The nanoparticle and nanocomposite were analyzed by SEM, which shows size of 72.4 and 125 nm respectively .The nanoparticle and nanocomposite were found to be nano rod, hexagonal in 2D shape respectively. The nanocomposite did not show bactericidal activity against Gram-negative or Gram-positive bacteria. The pathogens detected by UV light after incubation for 1hr.

Keywords: Nanoparticle; nanocomposite; fluorescence, 4-methylumbellipheryl-?-Dglucuronide (MUG).