Abstract

ECO-FRIENDLY SYNTHESIS OF CHITOSAN-PVA-SILVER NANOCOMPOSITE FOR SUSTAINED RELEASE OF ASPIRIN

Devipriya R. Majumder*, Pruthvi Chavan, Deepali Bankar, Kounsar Nabi Sheikh, Zoya Khan and Asma Parkar

Abstract

The present study explores the eco-friendly synthesis of Chitosan- PVA-Silver (CPSNP) nanocomposite in view of their increasing biomedical applications. Synthesis of the nanocomposite was achieved using two different approaches: “All in situ” by casting method and spray drying and “in situ polymerization with encapsulation of ex situ formed nanoparticles” by tray and spray drying. The reduction of silver ions was achieved using Chitosan, PVA(Poly Vinyl Alcohol) and by the cell free extract of White Rot fungi. The synthesis of silver nanoparticles was characterized by UV-Visible spectroscopy in the range of 300-600nm and a Surface Plasmon Resonance peak was observed at 410-430nm. FTIR was used to chemically confirm the presence of silver nanoparticles in the cross-linked Chitosan-PVA matrix and analyze the reducing groups in the cell free extract of White Rot fungi. The swelling capacity and anti-microbial activity was determined in view of their wound healing applications and demonstrated significant effects against E. coli, Pseudomonas, Staphylococcus and Klebsiella. Drug loading efficiency (LE), encapsulation efficiency (EE) and in vitro drug release of aspirin as the model drug from the nanocomposite was studied to determine its efficiency as a controlled drug delivery system. Aspirin loading (LE) and encapsulation efficiency (EE) was found out to be 16.8%, 84% respectively. The in vitro drug release was estimated to be 15% in 0.1N HCl in 15min and 30.25% in 90mins in phosphate buffer saline pH 7.4.

Keywords: Silver Nanoparticles, White Rot fungi, Chitosan, Poly Vinyl Alcohol, in situ and ex situ method, antimicrobial property, Aspirin, loading efficiency (LE),encapsulation efficiency (EE),in vitro drug release.