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Abstract

SCREENING OF ENDOPHYTES OF GLORIOSA SUPERBA FOR THEIR PLANT GROWTH PROMOTING POTENTIAL AND OPTIMIZATION OF INDOLE-3-ACETIC ACID PRODUCTION

Sneha Ogale*, Aditi Kulkarni, Sonali Nichit and Divya Shah

Abstract

Depletion of soils due to their repeated use in farming without replenishment leads to low productivity. This problem can be addressed with the application of fertilizers. Whereas chemical fertilizers are costly and can lead to deterioration of soil health, biofertilizers can prove to be an attractive alternative. Endophytes are endosymboints which enhance the growth of host plants by carrying out plant growth promoting (PGP) activities such as nitrogen fixation, phosphate solubilization and synthesis of auxins like indole-3-acetic acid (IAA). Hence they have a good potential in the development of biofertilizers or in the synthesis of auxins for use in horticulture/ agriculture. With this in mind, nine bacterial endophytes isolated from the rhizomes of Gloriosa superba (Kalalaavi) during a previous study, were used. They were screened for a) their ability to produce IAA in Nutrient broth supplemented with 5mg/ mL tryptophan (Salkowski’s reagent was used to detect IAA); b) for nitrogen fixation by their ability to grow in Jensen's nitrogen-free medium and c) phosphate solubilisation as those cultures forming clear zones around their colonies on Pikovskaya’s agar medium. All nine isolates demonstrated the ability to fix nitrogen, four to produce IAA, and two to solubilize phosphate. Optimization of parameters for the production of IAA was carried out for the selected isolates in Nutrient broth supplemented with tryptophan, by varying one factor at a time viz. pH, temperature, period of incubation and concentration of tryptophan added to the medium. The highest producer of IAA, isolate KV-P, produced 193.33 μg/ml of IAA under optimized conditions of pH 7.0, 30°C and 96 hours of incubation in Nutrient broth supplemented with 5mg/mL tryptophan.

Keywords: Gloriosa superba endophytes, IAA, optimization, phosphate solubilization, Nitrogen fixation.


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