Abstract

GREEN SYNTHESIS OF SILVER NANOPARTICLES USING GNIDIA GLAUCA AND COMPUTATIONAL EVALUATION OF SYNERGISTIC POTENTIAL WITH ANTIMICROBIAL DRUGS

Sandesh S. Shinde, Komal A. Joshi, Sumersing Patil, Shailza Singh, Rohini Kitture, Jayesh Bellare and Dr. Sougata Ghosh*

Abstract

Phytochemical diversity of medicinal plants makes them most suitable for synthesizing and stabilizing metal nanoparticles used for biomedical applications. Herein, we report an environmentally friendly, rapid and extremely efficient green synthesis of silver nanoparticles (AgNPs) using Gnidia glauca flower, leaf and stem extract. Rapid synthesis was monitored by UV–vis spectroscopy and characterized by high resolution transmission electron microscopy (HRTM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and X-ray diffraction (XRD) which revealed monodisperse AgNPs in a size range of 10 to 100 nm were predominant. Fourier transform infrared (FTIR) spectra indicated the mechanism of the synthesis and the role of the functional groups of the diverse phytochemicals involved in bioreduction and stabilization process. Further, the antibacterial synergy between AgNPs and diverse groups of antibiotics like β-lactam, chloramphenicols, aminoglycosides, tetracyclines, and glycopeptides showed greater bactericidal efficiency when combined with nanosilver. Streptomycin and chloramphenicol in combination with AgNPs showed marked synergism as compared to others that supported our observed results in-vitro that was further studied by computational analysis.

Keywords: Gnidia glauca, silver nanoparticles, high resolution transmission electron microscopy, X-ray diffraction, fourier transform infrared spectroscopy, antibacterial synergy.