Abstract

TO STUDY PHENOTYPIC AND MOLECULAR CHARACTERISTICS OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE ISOLATED FROM TERTIARY CARE HOSPITAL

Pranav Tambe*, Madhura Naik, Dr. Vrushali Thakkar, Dr. (Brig) K. K. Lahiri and Dr. V. R. Sapre

Abstract

Production of Metallo-Beta-lactamase (MBL) enzyme is most important resistant mechanism against carbapenems. Metallo-Beta lactamases comes under Ambler class B. This enzyme breaks the Betalactam ring of the drug thus making the drug inactive. New Delhi Metallo-Beta-lactamase is an enzyme which is active against compounds that possess Beta-lactam ring like Penicillins, Cephalosporins, Carbapenems except aztreonam and colistin. The gene that encode for NDM-1 is known as blaNDM-1 and has been identified on bacterial plasmids and chromosomes. Carbapenems are one of the very few therapeutic agents available for treating multidrug resistant infections caused by Enterobacteriaceae however, carbapenemases are increasingly being reported, with the latest threat being New Delhi metallo-β-lactamase (NDM-1). Transmission of plasmid carrying resistant gene has increased the incidence of multidrug resistance. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.

Keywords: Metallo-Beta lactamases, blaNDM-1, Carbapenems.