THE CULTURABLE ORAL MICROFLORA OF HEALTHY INDIVIDUALS AND PATIENTS WITH DENTAL INFECTIONS
Kulkarni Snehal*, Peerzade Alfiya Arif, Neha Kumari, Bagdadi Maryam and Shaikh Uzma
Abstract
Background: It has been recently shown that microbial life plays a
key role in regulating a broad range of systems, including the human
body. Studies on the characterization of healthy versus unhealthy
human microflora in different body sites and disease condition have
demonstrated a high potential for microbial community profiling
techniques in the diagnosis and treatment of diseases. Objective: To
study culturable oral microflora of healthy individuals with no dental
infections and patients with dentals infections. Materials and
Methods: Samples such as teeth swab, tongue swab and oral wash
were collected from individuals with no dental infections and patients
with dental. Standard microbiological procedures were followed in the
isolation of cultures from samples. Conventional biochemical methods
were used to identify the microorganisms. The characterization of
isolates involved in the determination of biofilm forming potential and
assessment of synergistic antimicrobial action of some herbal extracts against the oral
species. Results: This study evaluated the prevalence of Lactobacillus, Micrococcus,
Staphylococcus and Candida species in the oral samples. Microbial diversity of tongue swab
samples was higher as compared to other samples. Amongst the organisms isolated from
various samples, Candida spp. showed highest production of biofilm. Herbal extracts were
tested for biofilm inhibition and disruption assays and were seen to inhibit 30 to 70% and
disrupt 26 to 72% of biofilm formation. Conclusion: The present study focused on studying
the culturable oral flora of healthy individuals with no dental infections and patients with
Keywords: dental problems along with a suggestion for use of herbal extracts for non-formation of biofilms.
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