Abstract

THE CULTURABLE ORAL MICROFLORA OF HEALTHY INDIVIDUALS AND PATIENTS WITH DENTAL INFECTIONS

Kulkarni Snehal*, Peerzade Alfiya Arif, Neha Kumari, Bagdadi Maryam and Shaikh Uzma

Abstract

Background: It has been recently shown that microbial life plays a key role in regulating a broad range of systems, including the human body. Studies on the characterization of healthy versus unhealthy human microflora in different body sites and disease condition have demonstrated a high potential for microbial community profiling techniques in the diagnosis and treatment of diseases. Objective: To study culturable oral microflora of healthy individuals with no dental infections and patients with dentals infections. Materials and Methods: Samples such as teeth swab, tongue swab and oral wash were collected from individuals with no dental infections and patients with dental. Standard microbiological procedures were followed in the isolation of cultures from samples. Conventional biochemical methods were used to identify the microorganisms. The characterization of isolates involved in the determination of biofilm forming potential and assessment of synergistic antimicrobial action of some herbal extracts against the oral species. Results: This study evaluated the prevalence of Lactobacillus, Micrococcus, Staphylococcus and Candida species in the oral samples. Microbial diversity of tongue swab samples was higher as compared to other samples. Amongst the organisms isolated from various samples, Candida spp. showed highest production of biofilm. Herbal extracts were tested for biofilm inhibition and disruption assays and were seen to inhibit 30 to 70% and disrupt 26 to 72% of biofilm formation. Conclusion: The present study focused on studying the culturable oral flora of healthy individuals with no dental infections and patients with

Keywords: dental problems along with a suggestion for use of herbal extracts for non-formation of biofilms.