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Abstract

VALIDATED STABILITY INDICATING MICROBIAL AGAR ASSAY METHOD FOR DETERMINATION OF PRULIFLOXACIN

Sweety Kanolkar*, Teja Walke, Yogita Sardessai

Abstract

The development of a specific agar diffusion bioassay for determination of Prulifloxacin is been carried out using the strain Salmonella typhimurium ATCC 23564 as a test organism. The validation of the method showed good linearity, precision and accuracy. Drug sample exposed to UV radiation (254nm), acid hydrolysis, alkaline hydrolysis, thermal stress were used to determine the specificity of the bioassay. This study demonstrates the validity of proposed bioassay, which will allow reliable quantification of prulifloxacin in dosage form and can be used as an alternative method for prulifloxacin analysis in stability studies.

Keywords: Prulifloxacin, agar diffusion bioassay, Salmonella typhimurium ATCC 23564, validation, stability.


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