Abstract

OPTIMIZATION OF NUTRIONAL FACTORS FOR THE PRODUCTION OF CYCLODEXTRIN GLYCOSYLTRANSFERASE BY A NOVEL MUTATED BACILLUS SP TPR71HNA6 USING PLACKETTBURMANN DESIGN

Ravinder Kashipeta

Abstract

The cyclodextrin glycosyltransferase (CGTase) enzymes are the most important group of commercially produced enzymes. The production of CGTase was optimized using a newly isolated mutated Bacillus sp. TPR71HNA6. The fermentation variables were selected and optimized via Plackett–Burman design. Eleven nutrients belonging to three categories viz.; nitrogen sources, carbon sources and mineral sources were screened. This design screens n variables in n-1 number of experiments. The results showed that the CGTase production varies from 18 to 68U/mL indicating that the selected compounds showed significant effect on the enzyme production. From these results it was also observed that yeast extract has highest significant than all other variables followed by starch, peptone and urea. Glucose has the least effect on the CGTase production by mutated Bacillus sp. TPR71H NA6.

Keywords: CGTase, Plackett-Burman Design, Optimization, Bacillus sp. TPR71HNA6.